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  • PYQs (GS & Optional)
  • (e) Recombinant DNA technology, plasmid, cosmid, artificial chromosomes as vectors, transgenic, DNA cloning and whole animal cloning (principles and methods).

    • Recombinant DNA technology
      • With the help of a diagram, explain the constitution of a cosmid and mention its advantages over a plasmid in recombinant technology.
      • What do you mean by a genetic clone? Describe a protocol of cloning of a recombinant DNA.
      • Illustrate the steps of constructing a recombinant cDNA from an mRNA.
      • Discuss the applications of recombinant technology in human health.
      • What are the various vectors used in genetic engineering? Write and compare the protocol for each.
      • (b) What are the various vectors used in genetic engineering? Write and compare the protocol for each.
      • What is recombinant DNA technology? How is it done? Discuss its applications.
    • Plasmid, Cosmids, Artificial chromosomes as vectors
      • Give schematic organization of cosmid. Why is it preferred over plasmid for gene cloning?
      • With the help of a diagram, explain the constitution of a cosmid and mention its advantages over a plasmid in recombinant technology.
      • Explain the distinctive features of plasmid and Cosmids vectors used in recombinant DNA technology.
      • Discuss the role of Cosmids and artificial chromosomes as vectors in recombinant DNA technology.
      • Plasmid and Cosmids.
      • Artificial and Natural Classifications.
      • Plasmids and Cosmids as vectors.
    • Transgenic, DNA cloning, Whole animal cloning (principles and methods)
      • Describe the principle and method of whole animal cloning.
      • Describe the principle and method of whole animal cloning.
      • What do you mean by a genetic clone? Describe a protocol of cloning of a recombinant DNA.
      • Describe the sequence specific DNA binding proteins.
      • Define cloning. Explain somatic cell nuclear transfer method with suitable examples.
      • Explain ‘Whole animal cloning' by giving suitable examples. Enumerate the steps involved in whole animal cloning.
      • Differentiate between principles and methods of DNA cloning and Whole animal cloning.
      • Differentiate between principles and methods of DNA cloning and Whole animal cloning.
      • What are transgenic animals? Describe their importance in research.
      • Ribozyme technology and gene cloning.
      • Various forms of DNA.
      • What is gene cloning? Give an account of standard vectors used in gene cloning.
      • Cloning.
  1. PYQs and Practice Questions
  2. UPSC Optionals
  3. Zoology Optional
  4. Genetics
  5. (e) Recombinant DNA technology, plasmid, cosmid, artificial chromosomes as vectors, transgenic, DNA cloning and whole animal cloning (principles and methods).
  6. Plasmid, Cosmids, Artificial chromosomes as vectors

Give schematic organization of cosmid. Why is it preferred over plasmid for gene cloning? (UPSC 2022, 10 Marks, )

कॉस्मिड की योजनाबद्ध संरचना दें। जीन क्लोनिंग के लिए इसे प्लास्मिड पर क्यों प्राथमिकता दी जाती है? (Give schematic organization of cosmid. Why is it preferred over plasmid for gene cloning?)
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With the help of a diagram, explain the constitution of a cosmid and mention its advantages over a plasmid in recombinant technology. (UPSC 2022, 8 Marks, )

आरेख की सहायता से, एक कॉस्मिड की संरचना को समझाएं और पुनः संयोजक प्रौद्योगिकी में एक प्लास्मिड पर इसके लाभों का उल्लेख करें। (With the help of a diagram, explain the constitution of a cosmid and mention its advantages over a plasmid in recombinant technology.)
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Introduction
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Explain the distinctive features of plasmid and Cosmids vectors used in recombinant DNA technology. (UPSC 2018, 20 Marks, )

रिकॉम्बिनेंट डीएनए प्रौद्योगिकी में उपयोग किए जाने वाले प्लास्मिड और कॉस्मिड वाहकों की विशिष्ट विशेषताओं की व्याख्या करें। (Explain the distinctive features of plasmid and Cosmids vectors used in recombinant DNA technology.)
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Introduction
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Discuss the role of Cosmids and artificial chromosomes as vectors in recombinant DNA technology. (UPSC 2011, 20 Marks, )

कोस्मिड्स और कृत्रिम गुणसूत्रों की पुनः संयोजक डीएनए प्रौद्योगिकी में वाहक के रूप में भूमिका पर चर्चा करें। (Discuss the role of Cosmids and artificial chromosomes as vectors in recombinant DNA technology.)
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Introduction
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Plasmid and Cosmids. (UPSC 2006, 20 Marks, )

प्लास्मिड और कॉस्मिड्स। (Plasmid and Cosmids.)
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Introduction
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Artificial and Natural Classifications. (UPSC 2005, 20 Marks, )

कृत्रिम और प्राकृतिक वर्गीकरण। (Artificial and Natural Classifications.)
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Introduction
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Plasmids and Cosmids as vectors. (UPSC 2001, 20 Marks, )

प्लास्मिड्स और कॉस्मिड्स को वाहक के रूप में। (Plasmids and Cosmids as vectors.)
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Introduction
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